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哈灵文献:miR-4504参与三角帆蚌珍珠层颜色的形成

热烈祝贺 上海海洋大学 -陈夏君 陈老师 文献发表成功

文献主题:

miR-4504参与三角帆蚌珍珠层颜色的形成

文献中引用的我司产品(加亮部分):

Mantle samples (500mg middle mantle and 500mg fringe

mantle from each mussel) were collected from groups A, N and P.

Samples were immediately frozen in liquid nitrogen and stored

at 80 C until use. Tyrosinase activity was measured using the

Tissue Tyrosinase Activity Assay Kit (HaLing, Shanghai, China), according

to the manufacturer's instructions. Mantle samples were

comminuted in liquid nitrogen and treated with lysis buffer. After

centrifugation, the supernatant was collected and mixed with

buffer and tyrosine. All samples were incubated at 25 C for 1 h.

Tyrosinase activity was measured using a microplate reader with

the dopachrome content at 475 nm (UV-3010; Shimadzu, Japan).

One-way ANOVAs were performed using SPSS 17.0 to determine

significant differences between groups. Data from the tyrosinase

activity experiments were expressed as the mean ± SD (n ¼ 6). A pvalue

of <0.05 was considered statistically significant.

文献链接:

链接: https://pan.baidu.com/s/1XoZQb0hjYlsTIV4aE1Zt-Q 提取码: ps5k

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