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哈灵文献:三角帆蚌的HcTyr和HcTyp-1

 热烈祝贺 上海海洋大学 -陈夏君 陈老师 文献发表成功

文献主题:

三角帆蚌的HcTyr和HcTyp-1

文献中引用的我司产品(加亮部分):

Themantle samples (0.5 g of themantle center and 0.5 g of themantle

pallial and mantle edge from each sample) from eight healthy

H. cumingii specimens (four purple mussels and four white mussels)

were collected and immediately frozen in liquid nitrogen and stored

at−80 °C until use. The tyrosinase activity was estimated using the tissue

tyrosinase activity assay kit (HaLing, Shanghai, China) according to

在−80°C下使用。酪氨酸酶活性用组织学方法测定

酪氨酸酶活性测定试剂盒(上海哈灵生物科技有限公司)

themanufacturer's instructions. In brief, the sampleswere comminuted

in some liquid nitrogen with a mortar and pestle and treated with the

lysis buffer. The solution was centrifuged, and the supernatant was collected

andmixed with buffer and substrate (tyrosine). Finally, the samples

were incubated at 25 °C for 60 min, and tyrosinase activity was

measured using a spectrophotometer with the dopachrome content at

475 nm (UV-3010; Shimadzu, Japan). Tyrosinase catalyzes a reaction

that converts tyrosine to dopachrome by hydroxylation and then to

dopaquinone. One unit of tyrosinase activitywas defined as the amount

of activity that produced 1 μmol of dopachrome per minute via the hydroxylation

of tyrosine by tyrosinase at 25 °C. One-way ANOVAwas performed

using SPSS 17.0 to determine whether there were any

significant differences. Data from the TYR activity experiments were

expressed as the mean± SD (n=5) values. Significance was accepted

at the level of p b 0.05.

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链接: https://pan.baidu.com/s/1NqU0epVjnHvB942Nth1Wxg 提取码: a24d

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