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“宸功生物”产品文献:指标C57BL/6J 小鼠腭突中嵴上皮细胞的原代细胞

   热烈祝贺 重庆医科大学 陈强老师  文献发表成功

 

文献主题:

腭部间充质干细胞生物学行为的比较

2,3,7,8-四氯二苯并对二恶英体外诱导的上皮细胞

 

以下是相关检测指标

dical University Animal Center were used in this study. They were
housed under constant conditions at a temperature of 22 ± 2 °C, hu
midity of 50 ± 10 %, and 12 -h light/dark cycle. Female mice were
crossed with males in the evening and checked for vaginal plugs the
next morning. Additionally, their weights were recorded (embryonic
day 0, E0). On gestation day 14 (GD14), pregnant mice with weight
gain greater than 2 g were sacrifificed for primary mesenchymal cells. All
experiments abided by the Institutional Ethical Guidelines for
Experiments with Animals.
2.2. Cells and medium
Epithelial cells and medium were obtained from Shanghai Haling
Biotechnology Company, Limited. Primary epithelial cells were derived
from the palatal tissue of GD14 embryos from C57BL/6 mice.
Immunoflfluorescence was performed to detect the expression of the
epithelial marker ZO-1, and microscopic morphology was used to
confifirm its shape. A specifific medium was used to grow the epithelial
cells.
Mesenchymal cells were obtained from the palatal tissue of GD14
embryos from C57BL/6 mice. The methods of mesenchymal cell ac
quisition and culture were according to the previous study (Feng et al.,
2013). Immunoflfluorescence was performed to detect the expression of
the mesenchymal marker vimentin, and microscopic morphology was
used to confifirm its form. The media were DMEM/F12 (1:1) basic (1×)
(Gibco, USA) with 10 % fetal calf serum (FBS, Sijiqing, China).

 

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